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Pellets were then resuspended in freezing media consisting of 40% DMEM, 10% DMSO, and 50% FBS, and aliquoted into barcoded cryovials, and cryopreserved

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The functional status of p53, in combination with other molecular markers, such as oestrogen and progesterone receptors, B-cell lymphoma 2 (Bcl-2), BCL2-associated X (Bax), and human epidermal growth factor receptor 2 (HER-2), provides insight into tumour proliferative capacity and replicative potential, making it a critical mechanistic hub in breast cancer biology (4345)

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