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[34] Low-retention tips and calibrated micropipettes (or sterile transfer tools aligned with your SOP) [24] Alcohol prep (e.g., 70% IPA) for exterior wipe-down of vial stoppers and surfaces (per lab SOP) Labels for concentration, solvent, date/time, and storage condition Single-use aliquot tubes (recommended to avoid repeated freezethaw cycles) [34] Reconstitution Calculations: mg mL (Target Concentration) For research workflows, document concentration clearly (mg/mL or g/mL)
doi: 10.7759/cureus.40948 42 JonesRMMercanteJWNeishAS
Further investigations are needed to elucidate the specific mis-spliced genes and proteins directly influenced by SF3B1 mutations together with the crosstalk between SF3B1 and MAPK pathway in tumorigenesis of MM, and the findings may provide a new perspective for targeted therapy
