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Metabolic phenotyping For the glucose tolerance tests, mice were fasted for 2 h (beginning at ZT0) and then administered glucose via oral gavage (2.5 g kg 1 body weight)

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Similarly, the in vivo administration of an equimolar dose of DTT (40 mm) instead of NAC to TNBS+ethanol rats failed to either increase GSH levels and protect against injury, suggesting that the beneficial effect of NAC was due to its role as a GSH precursor