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This is followed by the release of nesfatin-1 which stimulates cholecystokinin secretion leading to a rise in bile salts from gallbladder emptying, and the binding of Takeda P protein coupled receptor 5 (TGR5) onto the surface of the enteroendocrine cells, all of which eventually culminates in GLP-1 secretion [9]

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the column was washed with 20 ml equilibration solution, and elution was performed with a linear salt gradient from 0 to 250 mmol/kg KCI (20 mmol/kg citric acid, 45 % (w/w) ethanol, pH 3.5) followed by 40 ml of isocratic elution at 250 mmol/kg KCI

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