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For GSSG competition assay (Extended Data Figure 4l), the 2x reaction buffer consisted of 10LM [ 3 H]GSSG, 5mM GSSG, 150mM KCl, and 50mM HEPES (pH 7.4)

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Additional Systemic Benefits Animal research also suggests possible benefits of using GHK-Cu for the following applications: Lung protection in injury models DNA repair signaling Anti-cancer activity Anxiolytic and analgesic effects Preservation of gut barrier integrity These are much less conclusively proven in humans compared to the previous benefits, but they are biologically plausible based on known GHK-Cu signaling pathways

Purging the vial headspace with nitrogen or argon removes oxygen and dramatically reduces oxidation rates

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