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The paraffin-embedded sections were dehydrated with xylene, absolute ethanol, 95% alcohol, 80% alcohol, 70% alcohol, and distilled water

For ESCC induction, 4-nitroquinoline 1-oxide (Sigma, Shanghai, China) was dissolved in propylene glycol (Sigma) and diluted in the drinking water at a final concentration of 100 g ml 1

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Pyrroloquinoline Quinone (PQQ): Has potent antioxidant effects that reduce oxidative stress and support healthy aging

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