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pDD162 ( Peft-3::Cas9 ) was injected into wild-type animals at 50 ng/L with a targeted gRNA and dpy-10 gRNA in plasmid vectors at 25 ng/L, the targeted donor oligonucleotide (gRNA1: GGAGGATATAGTGATCATCGAGG, gRNA2: CCCCCTTTCACGTGGGACACGTC), and the dpy-10(cn64) donor oligonucleotide at 500 nM

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Meyer T, Husch M, van den Berg E, et al

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