Western blot and real-time PCR Real-time PCR was performed using a Roche LightCycler 480 detection system (Roche, Basel, Switzerland)
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Significant positive correlations were found between the intrafollicular concentration of MI and the level of estradiol in the follicular fluid, the cleavage rate of fertilized oocytes, the stage of embryos ( 4 cells) and embryo quality (grade)
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Several biological factors have been found to contribute to this association, including altered estrogen metabolism, insulin resistance, and the promotion of inflammation, fibrosis, and angiogenesis [46,47,48,49]
