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The final measurement at the end of this step reflects the total content of GSH (free GSH + GSSG) in the samples, to calculate the GSSG content, the free GSH content must be subtracted from the total GSH content

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It has been implicated in the regulation of enzyme activities, in DNA synthesis, in maintaining the viability of chloroplasts and mitochondria, in the protection of nitrogenase from inhibition by oxygen, and in the regulation of gene expression.* Glutathione levels decrease naturally with age, as well as with certain stressors, including exercise, smoking, and pollution

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