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Glycocalyx degradation usually begins in the SIRS stage, when proinflammatory factors (TNF-, IL-1) activate endothelial cells and directly destroy the components of the glycocalyx (such as HS, HA) by activating MMPs [23]

HPLC coupled with detectors like UV or fluorescence is also a well-established and reliable method for quantifying specific vitamins in CF, utilizing similar extraction procedures prior to chromatographic separation and detection (Francalanci et al., 2023)

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