Trapped peptides were then gradient-eluted and separated on an Acquity CSH C18 NanoEase column (75 m 25 cm, 1.7 m particle size) (Waters Corporation, Milford, Massachusetts, United States) at a flow-rate of 0.3 L min 1 with a gradient of 640% B (A: 0.1% FA
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[DOI] [PMC free article] [PubMed] [Google Scholar] Thiele TE, Seeley RJ, DAlessio D, Eng J, Bernstein IL, Woods SC, Van Dijk G, 1998
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& Echaniz-Laguna, A