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This pattern aligns with B12 pharmacokinetics: serum levels peak 8-12 hours post-injection, tissue saturation occurs over 48-72 hours (Andrs et al., Journal of Blood Medicine , 2018)

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Whilst taking the time to test for GST and/or GT tumor expression may slightly delay treatment, it will ensure that GSH-conjugates anticancer prodrugs will be administered to the patients that are more likely to respond