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Moreover, to show that this potential hypoallergenic fish was safe for human consumption, the EDTA retained in the fish muscle was assessed by high-performance liquid chromatography (HPLC), and the concentration was below the aforementioned values established by the EU, for the two species ( Follow-up IgE-reactivity studies using patients sera with a confirmed clinical reactivity to these species should be performed to improve and corroborate the reported results, since cases of monosensitivity to specific fish species or single parvalbumin isoforms are common ( in vitro digestibility tests, skin-prick tests, basophil histamine release assays and at the very end double-blind placebo-controlled food challenges (DBPCFC)