Transient transfection of HEK-293 cells with these plasmids was performed using Lipofectamine and Plus reagent according to the manufacturers protocol (Thermo Fisher Scientific)
In parallel, glycine, another major component of collagen, is synthesized from serine, which is derived from the glycolytic intermediate 3-phosphoglycerate via the enzymes phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase 1 (PSAT1), and phosphoserine phosphatase (PSPH), and subsequently converted by serine hydroxymethyltransferase 1/2 (SHMT1/2) [17, 18]
Author contributions ZX, JT, and JZ made the idea and experimental design of this study
J Gastrointest Surg Off J Soc Surg Aliment Tract 13(2):312317
This neovascularization phenomenon was consistently observed in murine full-thickness burn injuries (Fig
A Randomized, Double-Blind, Placebo-Controlled, Crossover Study Examining the Hormonal and Vitality Effects of Ashwagandha (Withania somnifera) in Aging, Overweight Males