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Hormone and cAMP measurement For the measurement of plasma GLP-1, blood samples were collected in ice-cooled BD Microtainer blood collection tubes coated with K2EDTA (36784, BD) followed by addition of 50 M DPP-IV inhibitor (DPP4, Merck/millipore), while culture medium was collected plus 1% DPP-IV inhibitor and centrifuged for 5 min at 4 C at 12,000 g

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Just several months later that estimate was $130bn